Name: CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)
Price: 414.00 USD

- Overview
  - This is an engineered T7 RNA polymerase variant (low dsRNA) derived from the wild-type T7 RNA polymerase and produced in Escherichia coli. It significantly reduces the production of double-stranded RNA (dsRNA) while efficiently incorporating cap analogs, and exhibiting highly efficient in vitro transcription (IVT) comparable to the wild-type T7 RNA polymerase. It catalyzes the 5'→3' synthesis of RNA on double-stranded DNA from its T7 promoter sequence (5'-TAATACGACTCACTATAG*-3') and uses NTPs as substrates.
    
    Note: G* is the first base of the RNA transcript.
    
    Feature
    • dsRNA level lower to about 1/100000
    • Comptiable with Trilink CleanCap AG
    • High yields comparable to WT
    • Lower Cap input
    • Animal origin-free (AOF)
    
    Unit Definition: the amount of enzyme required to incorporate 1 nmol of [³H] GMP into the acid-insoluble precipitate within 1 hour at 37°C and pH 8.0 is defined as 1 unit.
    
    Please contact us at for specific academic pricing.
- Properties
  - Purification
    
    Protein Purity≥95%
    
    Storage
    
    The products are shipped with dry ice and can be stored at -15°C to -25°C for one year.
    Storage Buffer: 50 mM Tris-HCl, 1 mM EDTA, 10 mM DTT, 100 mM NaCl, 0.1% Triton X-100，50% (v/v) glycerin，pH7.9 at 25°C
    
    Expression system
    
    Recombinant E. coli with T7 RNA Polymerase gene
    
    Endotoxin
    
    <20 EU/mg
    
    Activity
    
    250-300U/μL
    
    * For Research Use Only.

MSDS

Documents

Manual

CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

CleaScrip™ T7 RNA Polymerase (low dsRNA, 250 U/μL)

Documents

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