Name: Ubiquitin - ¹⁵N microcrystals
Price: 921.00 USD

- Overview
  - MW= 8.6 kDa calculated. Full length Ubiquitin cloned from human cDNA, expressed in E. coli. The protein consists of the human ubiquitin (residues 1-76, UniProtKB accession P0CG47).
    
    · Characteristics
    Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2mM.
    
    Labeling: 15N
    Physical state: Microcrystalline
    NMR Standard: Bulk sample
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Human
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
    
    Formulation
    
    1 mg/mL solution in phosphate buffer 50 mM pH 7, NaN3 0.02%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1490 M^-1cm^-1 calculated).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
    NMR USE: after the experiments return the sample at 4°C.
    
    Molecular Weight
    
    8.6 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    10 20 30 40 50
    MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL
    60 70
    EDGRTLSDYN IQKESTLHLV LRLRGG
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - Ubiquitin-binding domains
    Assignment of the backbone resonances for microcrystalline ubiquitin

Ubiquitin - ¹⁵N microcrystals

Ubiquitin - ¹⁵N microcrystals

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