TriRNA Pure Kit without DNase

- Overview
  - The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or other phenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Purify total RNA in 10 minutes!
    No chlorophorm phase separation
    No isopropanol RNA precipitation
    No phenol carryover
    Compatibility: homogenize samples with GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
    Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma), up to 5 x 10⁶ cultured cells, 10-50 mg of tissue, 1 x 10⁹ bacterial cells, 20-50 mg of plant tissue
    A cost effective phenol, guanidine isothiocyanate solution plus spin column system
    High quality RNA: A260/A280 >1.8, A260/A230 >1.8
    Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
    Membrane: glass fiber membrane optimized for total RNA extraction
    Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
    
    Storage
    
    dry at room temperature (15-25°C)
    
    * For Research Use Only
- Applications
  - Application
    
    Total RNA Extraction
    
    Application Description
    
    cDNA Library Construction, Cloning, RT-PCR (Endpoint), Real-Time PCR, Nuclease Protection Assays, Northern Blotting

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PROTOCOL

TriRNA Pure Kit without DNase

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