Tribo™ Human TGF-β1 ELISA Kit (TBS3085)

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are pipetted into the wells, and incubated with biotin-conjugated detection antibody (Detection A) specific for human TGF-β1. After incubation, the plate wells are aspirated without washing. Then, incubate with Streptavidin- HRP (Detection B). Following a wash to remove any unbound antibody and samples, an ultra-sensitive TMB substrate solution is added to the wells for color development. The color intensity is in proportion to the amount of TGF-β1 bound in the initial step. The intensity of the color is measured by plate read at 450 nm.

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