TPEN

- Overview
  - TPEN is a highly selective chelator of transition metal cations because the pyridine groups act as soft electron donators. TPEN is thus utilized for masking transition metal cations in fluorescent calcium monitoring. The masking function of the zinc cation is important for canceling the Fluorescent effect. TPEN is water-soluble and cell membrane permeable. Maximum wavelength is 260 nm. The physical properties of TPEN are as follows: pKa1=10.27, pKa2=3.32, pKa3=4.85, and pKa4=7.19; LogKMn=10.27, logKFe=14.61, logKZn=15.58, logKMg=1.7, and logKCa=4.4.
    
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- Properties
  - Categories
    
    Intracellular Fluorescent Probes
    
    Alternative Name
    
    N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine
    
    CAS Number
    
    16858-02-9
    
    Molecular Formula
    
    C26H28N6
    
    Molecular Weight
    
    424.54
    
    Appearance
    
    white or slightly yellow crystals
    
    Purity
    
    ≥98.0% (Titration)
    
    Storage
    
    ambient temperature
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Reference
  - 1) G. Anderegg, et al., Pyridinderivate Als Komplexbildner VIII Die Herstellung Je Eines Neuen Vier- und Sechszahnigen Liganden. Helv Chim Acta. 1967;50:2330-2332.
    
    2) G. Anderegg. et al., Pyridinderivate als Komplexbildner. XI). Die Thermodynamik der Metallkomplexbildung mit Bis-, Tris- und Tetrakis[(2-pyridyl) methyl]-aminen. Helv Chim Acta. 1977;60:123-140.
    
    3) P. Arslan, et al., Cytosolic Ca2+ Homeostasis in Ehrlich and Yoshida Carcinomas A, New Membrane-Permeant Chelator of Heavy Metals Reveals That These Ascites Tumor Cell Lines Have Normal Cytosolic Free Ca2+. J Biol Chem. 1985;260:2719-2727.
    
    4) T. Tatsumi, et al., Hypochlorous Acid Mobilizes Intracellular Zinc in Isolated Rat Heart Myocytes. J Mol Cell Cardiol. 1994;26:471-479.
    
    5) I. Sakabe, et al., Induction of Apoptosis in Neuro-2A Cells by Zn2+ Chelating. Cell Struct Funct. 1998;23:95-99.

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TPEN

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