TNP (Trinitrophenol) Coated Plates

TNP Coated 96 Well Plates are offered with 12 breakable strips of 8 wells assembled on single well holding frame. Clear breakable strips are manufactured in pure polystyrene with low fluorescence and can be used for ELISA assays. These strips are designed to be easily breakable by the user and repositioned in the frame; the radius edged inner bottom of the wells improves the efficiency of washing. The TNP Coated 96 Well Breakable Strip Plates comply with SBS standards and the design assures a good performance in automatic processing plant.

· Plates are pre blocked with proteic blocking
· Recommended working volume of 50 to 100 μl
· Each coated plate is packed in a single barrier bag with desiccant
· Every single unit contains 5 plates
· White and black TNP Coated 96 Well Breakable Strip Plates can be used for Luminescence and Fluorescence assays are AVAILABLE BY ORDER ONLY.

Surface:
TNP coated surface on the TNP Coated 96 Well Breakable Strip Plates can be used as biomarkers of immunotoxicity, assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing and determination of immune status relative to controls.

Please contact us at for specific academic pricing.

Background

Coated Surface for Immunotoxicity ELISA Test is ideal for setting up ELISA IgM and IgG assays to be used as biomarkers of immunotoxicity.
Immunotoxicity is the adverse effected of the immune system caused by exposure to certain substances (chemicals, environmental, drugs, etc.) that are capable of altering specific immune functions, causing an undesirable effect to the organism.

Immunotoxicity analysis play a fundamental role in studying the development of new drugs because hypersensitivity reactions are the most frequent immunotoxic effects of drugs, both immune-mediated (allergic) and non-immune-mediated (pesudo-allergic) reactions.

A drug, before its release, is subjected to different phases of study: “in vitro” and “in vivo” studies on animals (pre-clinical experimentation) and so-called phase 1, phase 2 and phase 3 studies performed on humans (clinical experimentation).

In preclinical trials, to assess whether a drug can cause immunosuppression problems to the immune system, animals are immunized with specific antigens such as: KLH (Keyhole Limpet Hemocyanine), SRBC (Sheep Red Blood Cells), Tetanus toxoid, DNP (Dinitrophenol), TNP (Trinitrophenol).

Coated Surfaces for Immunotoxicity Test are suitable for measuring antibodies by ELISA assays. This test allows you to collect samples in series compared to other methods and minimize the quantities of reagents and antisera needed in the test.

· Coated 96-Well Plates for Immunotoxicity ELISA Test main features
Biomat's Coated 96-Well Plates for Immunotoxicity have the following characteristics:
Ready to use
Manufactured under ISO:9001 guidelines
Post-coated (blocked) for low non specific binding and long-term stability
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Coated Surface for Immunotoxicity ELISA Test Products ensures:

Uniformity
All the 96-Well Coated for Immunotoxicity Test products show a CV% less than 5 when used as a sandwich of mouse IgG anti KHL (or anti DNP, anti TNP or anti Tetanus Toxoid) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

Storage and Stability
The microplates, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· KLH (Keyhole Limpet Hemocyanin)
The Californian giant keyhole limpet has a hemocyanin that has been recognized as a potent immunoactivator and therefore is widely used in research and clinical studies.

Keyhole limpet hemocyanin (KLH) from Megathura crenulata can be used 1) as a highly immunogenic antigen for assessment of immune competence of an organism or 2) as a carrier of low molecular mass peptide and haptens, such as oligosaccharides, gangliosides or (glyco) peptides, designed to facilitate antibody production.

Examples of applications for KLH Coated 96-Well Plates:
assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
determination of immune status relative to controls
quantifying and standardizing vaccines batches and protocols

· KLH Product Specifications
Coating
KLH is coated using 100 µl/well.

Binding capacity
Microplate was saturated with mouse IgG anti KLH at a concentration of 0.250 µg/ml (25 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

· DNP (Dinitrophenol)
Dinitrophenol is an hapten that can be attached to carrier proteins such as BSA or ovoalbumin to assess the impact of pharmacologic or genetic manipulations on the studied immune system. When this complex is injected into appropriate animal models, it produces an immune response which is measured as changes in the levels of anti DNP IgM and IgG.

Examples of applications for DNP Coated 96-Well Plates:

used as biomarkers of immunotoxicity
assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
determination of immune status relative to controls

· DNP Product Specifications
Coating
DNP-BSA is coated using 100 µl/well.

Binding capacity
Microplate was saturated with mouse IgG anti DNP at a concentration of 0.125 µg/ml (12.5 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

· TNP (Trinitrophenol)
Trinitrophenol is an hapten that can be attached to carrier proteins such as BSA or ovoalbumin to assess the impact of pharmacologic or genetic manipulations on the studied immune system.

In fact, when this complex is injected into appropriate animal models, produces an immune response which is measured as changes in the levels of anti TNP IgM and IgG.

Examples of applications for TNP Coated 96-Well Plates:

used as biomarkers of immunotoxicity
assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing
determination of immune status relative to controls

· TNP Product Specifications
Coating
TNP-BSA is coated using 100 µl/well.

Binding capacity
Microplate was saturated with mouse IgG anti TNP at a concentration of 0.250 µg/ml (25 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.

· Tetanus Toxoid
Tetanus Toxoid coated plates are ideal to set up assays to measure spectrophotometrically the level of anti-tetanus toxoid antibodies present in biological samples, e.g. ELISA tests.

Tetanus, commonly called lockjaw, is a serious bacterial disease that affects muscles and nerves. It is characterized by muscle stiffness that usually involves the jaw and neck that then progresses to involve other parts of the body. This disease is caused by neurotoxin from deep wound infection with Clostridium tetani.

Examples of applications for Tetanus Toxoid Coated 96-Well Plates:

assessing efficacy of vaccines
quantifying and standardizing vaccines batches and protocols

· Tetanus Toxoid Product Specifications
Coating
Tetanus Toxoid is coated using 100 µl/well.

Binding capacity
Microplate was saturated with mouse IgG anti Tetanus Toxoid at a concentration of 0.125 µg/ml (12.5 ng/well) in an ELISA format using goat anti mouse IgG-HRP as detector and TMB as substrate.