Name: TNF-alpha (human) (multimeric) (Recombinant)
Price: Inquiry

- Overview
  - Specific Binds to human and mouse TNF-R1 and TNF-R2. Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. MultimericTNF-alpha is a high activity construct in which two trimeric TNF-alpha ligands are artificially linked via the collagen domain of ACRP30. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-alpha (TNF-alpha (human) (multimeric) (rec.) (32-190048). Since the MultimericTNF-alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-alpha protein that can activate the TNF-R2. For TNF-R1 activation, either "normal" TNF-alpha or MultimericTNF-alpha can be used.
    
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- Properties
  - Protein Name
    
    MultimericTNF-alpha; ACRP30headless:TNF-alpha; ACRP30headless:TNFSF2; ACRP30headless:Tumor Necrosis Factor-alpha
    
    Source
    
    HEK293 cells
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human; Mouse
    
    Purification
    
    >=95% (SDS-PAGE)
    
    Storage
    
    Short Term Storage +4°C ; Long Term Storage-20°C ; After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0. 1% BSA should be used for further dilutions. Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
    
    Endotoxin
    
    <0.02EU/µg purified protein (LAL test; Lonza).
    
    Activity
    
    Activates human and mouse TNF-R1 and TNF-R2. Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells).
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    Functional Assay

TNF-alpha (human) (multimeric) (Recombinant)

TNF-alpha (human) (multimeric) (Recombinant)

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