Name: TNF a His Recombinant Protein
Price: Inquiry

- Overview
  - Tumor Necrosis Factor-a Human Recombinant His produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids fragment (77-233) and having a molecular mass of 18.3kDa with an amino-terminal hexahistidine tag. The TNF-alpha His is purified by standard chromatographic techniques. Tumor necrosis factor is a cytokine involved in systemic inflammation and is a member of a group of cytokines that all stimulate the acute phase reaction. TNF is mainly secreted by macrophages. TNF causes apoptotic cell death, cellular proliferation, differentiation, inflammation, tumorigenesis and viral replication, TNF is also involved in lipid metabolism, and coagulation. TNFs primary role is in the regulation of immune cells. Dysregulation and, in particular, overproduction of TNF have been implicated in a variety of human diseases- autoimmune diseases, insulin resistance, and cancer.
    
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- Properties
  - Protein Name
    
    TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 97.0% as determined by SDS-PAGE.
    
    Formulation
    
    TNF-a His is supplied in 1xPBS.
    
    Storage
    
    Lyophilized TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to reconstitute the lyophilized TNF-α in sterile 18M-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions. EC50 0.200-0.120ng/ml measured in a metabolic inhibitor actinomycin D assay using L-cells.

TNF a His Recombinant Protein

TNF a His Recombinant Protein

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