TGF b 3 HEK Recombinant Protein

TGF b 3 HEK Recombinant Protein

Catalog Number:
P001416858ABE
Mfr. No.:
Abe32-1774
Price:
  • Size:
    1 µg
    Quantity:
    Add to Cart:
      • Overview
        • TGF-b 3 Human Recombinant produced in HEK cells is a non-glycosylated homodimer, having a total molecular weight of 25kDa. The TGF-b 3 is purified by proprietary chromatographic techniques. Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule.

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      • Properties
        • Protein Name
          Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
          Source
          HEK293 cells
          Type
          Recombinant Proteins
          Purification
          Greater than 95% as obsereved by SDS-PAGE.
          Formulation
          The TGF-b 3 was lyophilized from 1mg/ml in 1xPBS.
          Storage
          Lyophilized TGF-b 3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-b 3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized TGF-b 3 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA. The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2) and is typically 0.1-0.5ng/ml.

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