T4 DNA Ligase

- Overview
  - T4 DNA Ligase is highly efficient, accurate and rapid enzyme designed for an efficient ligation of cohesive and blunt ended DNA insert into plasmid vectors in 5-15 minutes. It is based on the combination of T4 DNA Ligase with a Premium 5x Ligation Buffer. The ligation reaction mixture is used directly for bacterial transformation using conventional transformation procedures. It enables sticky end or blunt end DNA ligation in only 5 minutes at Room Temperature.
    
    Includes for 300 U:
    – 60 µL T4 DNA Ligase (5 U Weiss/µL)
    – 250 µL T4 DNA Ligase Buffer (5x)
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Related Enzymes
    
    Storage
    
    -20 °C.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application
    
    End-Point PCR
    
    Application Description
    
    Routine cloning procedure: blunt and cohesive double stranded DNA cloning.
    TA cloning.
    Joining of adaptors with blunt or cohesive ends.
- Reference
  - Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
    Lago-Docampo, M., Tenorio, J., Hernández-González, I., Pérez-Olivares, C., Escribano-Subías, P., Pousada, G., … & Valverde, D. (2020). Characterization of rare ABCC8 variants identified in Spanish pulmonary arterial hypertension patients. Scientific Reports, 10(1), 1-13.

Datasheet

MSDS

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Brochure

Manual

T4 DNA Ligase

Documents

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