Streptactin 4FF Chromatography Column, 1 mL Streptactin (Ⅱ) tagged protein purification prepacked column

Streptactin 4FF Chromatography Column, 1 mL Streptactin (Ⅱ) tagged protein purification prepacked column

Catalog Number:
ACC1505783YEA
Mfr. No.:
20494ES03/08
Price:
$250
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      • Overview
        • Strep-Tag II is a small tag of 8 amino acids (WSHPQFEK). Since the tag is small, only about 1.06 kDa, it does not affect the structure and function of the fused protein, so there is no need to remove the tag. It has been widely used due to its high specificity, high purity in single-step purification, mild purification conditions, and fusion of both ends of the protein. Twin Strep-tag II is a sequence of two Strep-tag II sequences (connected by internal amino acids). This tag can be purified gently and quickly like Strep-tag II.

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      • Properties
        • Other Properties
          Matrix: Highly cross-linked 4% agarose gel
          Ligand: Streptactin
          Particle size: 45-165 µm
          Loading capacity: 3-5 mg Twin Strep-Tag II protein/mL Matrix
          Maximum flow rate: 300 cm/h
          Storage buffer: 1× PBS with 20% ethanol
          Storage
          Store at 2~8°C, valid for 2 years.
      • Applications
        • Application Description
          Streptactin is one of the most stable proteins. It is coupled to highly cross-linked 4% agarose microspheres and can be used in various expression systems, including purification of proteins containing Strep-Tag II tags in baculovirus, mammalian cells, yeast and bacteria. The resin can also be used for purification of Twin Strep-Tag II tags.
          Streptactin 4FF Chromatography Column is a medium-pressure prepacked column with Streptactin Agarose Resin 4FF as filler. The specification is 1 mL. The prepacked column has a standard interface and can be adapted to various commercial medium-pressure chromatography systems, such as ÄKTA, etc., for easy operation by customers.

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