SP Bestarose HP - Strong Cation Exchange Resins

SP Bestarose HP - Strong Cation Exchange Resins

Catalog Number:
CM01502214BES
Mfr. No.:
AI0080-AI203116
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      • Overview
        • Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
          IEX resin is composed of three parts:
          (1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
          (2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
          (3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
          SP Bestarose HP (high performance) is a strong cation exchange resin formed by coupling sulfopropyl groups on fine-grained high-resolution agarose microspheres.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          Strong Cation Exchange Resins
          Other Properties
          Appearance: White slurry
          Matrix: Cross-linked agarose, 6%
          Functional group: Sulfopropyl
          Particle size+: 24~44μm (the average particle size is 34μm)
          Ionic capacity: 150~200μmol H+/mL packed resin
          Dynamic binding capacity ++: ~ 70 mg BSA/mL resin
          Max. pressure: 0.3MPa
          Chemical stability: Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,6M GuHCl, 30%isopropyl alcohol, 70% ethanol. Avoid contact with oxidizing agents, cationic detergents.
          pH stability: 3~14 (CIP), 4~13 (working)
          Operating temperature: Operating temperature: 2~40°C, do not freeze.
          Storage++++: 2~30°C, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc
          Recommended flow velocity: 60-150cm/h

          + Average particle size is the accumulated resin particle size of packing volume distribution.
          ++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load at 100mM NaHAc pH9 flow velocity of 1mL /min,
          +++1M NaOH and 1M HAc only be used for cleaning.
          ++++2% benzyl alcohol is only used for international transport or special requirements from customer.

          * For Research Use Only

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