SmartAmp® Amplification Technology Reagent Kit

SmartAmp® is an isothermal DNA amplification technology for rapid detection with extreme fidelity.
Unlike other amplification methods, the complete suppression of background can be achieved when properly optimized. SmartAmp requires no DNA purification, and displays with the high sensitivity of single-nucleotide precision, enabling identification of single nucleotide polymorphisms (SNPs), mutations, and other DNA targets.

Principle of the SmartAmp® method
The SmartAmp® method is an isothermal nucleic acid amplification method. The technical feature of this method is that nucleic acids are amplified under isothermal conditions using asymmetric primer sets and DNA polymerase (Aac DNA Polymerase) with strand displacement reaction activity.
Primers used in the SmartAmp® method are designed to form a loop structure with the synthetic strand after elongation. This loop structure provides a single-stranded region to which the primers bind under isothermal conditions. The SmartAmp® method is characterized by its asymmetric primer design, which makes it easy to detect SNPs and genetic mutations.

Features of the Smart Amplification Process include:
Energy-saving: Isothermal amplification does not need thermocycling required (60°C incubation)
Time-saving: Unique primer design combined with a new enzyme with strand-displacement activity enables to shorten the reaction time less than 30 minuets.
Trouble-saving: The SmartAmp® chemistry enables easy one-pot reaction. Reaction mixture is prepared by quick and simple treatment of less than 1μL of whole blood samples.
Cost-saving: With the exclusive licenses, we manufacture and supply these cutting-edge gene-detection reagents kits in competitive prices.

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