Name: SH3 - ¹³C¹⁵N
Price: Inquiry

- Overview
  - MW = 7.2 kDa. Recombinant α-Spectrin SH3 domain (Spectrin, non-erythroid alpha chain, Fodrin alpha chain), cloned from cDNA from chicken and expressed in E. coli. The protein consists of the soluble SH3 domain of α-Spectrin from Gallus gallus (residues 965-1025). UniProtKB accession P07751.
    
    Labeling: 13C15N
    NMR Standard: Bulk sample
    Shipping in Dry Ice: no
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Chicken
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
    
    Formulation
    
    1 mg/mL solution in sodium citrate 10 mM pH 3.5, NaN3 0.02% , D2O 10%. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 15470 M^-1cm^-1 calculated).
    (Unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
    
    Storage
    
    -20°C. The protein is stable at 4°C for months. Avoid repeated freeze/thaw cycles.
    NMR USE: after the experiments return the sample at 4°C.
    
    Molecular Weight
    
    7.2 kDa
    
    Expression system
    
    E. coli
    
    Sequence
    
    970 980 990 1000
    M-DETGKE LVLALYDYQE KSPREVTMKK GDILTLLNST
    1010 1020
    NKDWWKVEVN DRQGFVPAAY VKKLD
    
    * This product is intended for research use or further manufacturing only.
- Reference
  - 1H and 15N NMR assignment and solution structure of the SH3 domain of spectrin: comparison of unrefined and refined structure sets with the crystal structure

SH3 - ¹³C¹⁵N

SH3 - ¹³C¹⁵N

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