Seplife® AG MOC/90 Multimodal Size Exclusion and Weak Base Anion Resin

Multimodal Chromatography - Agarose

Appearance: White to off white spherical beads
Type: Size exclusion and octylamine agarose
Matrix: Highly cross-linked 6% agarose
Ion exchange capacity (mmol/ml): 0.04-0.085
pH ligand fully charged: Positively charged at pH<10.5
Particle size range (μm): 45-165
pH stability: 3-13 (operational), 2-14 (CIP)
Chemical Stability: Stable in common aqueous solutions: 1M NaOH, 1M acetic acid. AVOID the use of Oxidizing agents, citrate buffers
Flow rate* (cm/h): max 1000cm/h
10% dynamic binding capacity (mg/ml)**: ≥15 (BSA)
Shipped as: 20% ethanol slurry

*Testing conditions: Chromatography column 16mm×200mm; column bed height 20cm; temperature 25°C; mobile phase water.
** Testing conditions: Binding buffer: Tris-HCl pH 8.0; Elution buffer: Tris-HCl +1.0M NaCl, pH 8.0. Sample: bovine serum albumin, Column 8mm*100mm, room temperature, Retention time 2 minutes.

Column packing
Column packing should be done according to standard operating procedures. It is important to ensure that each material is at its working temperature, and when possible, the chromatography media may be degassed before column packing.

Equilibration
Equilibrate the column with an appropriate 2-5 column volume buffer. Ensure the conductivity and pH of the effluent are the same as the equilibration buffer. The equilibration solution can be the buffer system used in conventional gel filtration and ion exchange chromatography, such as phosphate buffer and Tris buffer. It is recommended to avoid the use of acetate buffer systems, which reduce the adsorption capacity of the chromatography media.

Sample feeding
Typically, the target molecules follow the flow-through mode, and the impurities are adsorbed in the internal pores. The actual sample volume can reach 5-20 column volumes, mainly depending on the composition of the sample.
Rinse and regeneration
Rinse the column with equilibration buffer. The bound impurities can be removed by cleaning-in-place (CIP).

Cleaning-in-place (CIP)
To remove adsorbed contaminants and maintain product performance, periodic cleaning-in-place is required.
It is recommended, after each cycle, to rinse with 30% isopropanol solution containing 1.0 M NaOH or back rinse with 27% acetone solution. The contact time is 30-60 minutes, and the media can be temporarily stored in the solution for 15- 30 minutes to improve cleaning efficiency.
The concentration of NaOH, the type of organic solvent, the contact time and the volume of CIP can all be optimized according to the actual sample.
Disinfection
0.5-1.0M NaOH solution can be used to pass through the column, and the contact time is 1h to sterilize the used chromatography column.

Storage
Sealed and stored at 4~30°C (preservation solution is 20% ethanol) in a ventilated, dry and clean place, do not freeze.