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Overview
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The pseudovirus product uses the Spike protein from the NCBI Reference Sequence: NC_045512.2 as the pseudovirus spike protein, with specific mutationat the site: G75V, T76I, R246N, del247-253, L452Q, F490S, D614G和T859N. It is constructed using the HIV lentiviral packaging system and contains plasmids expressing GFP (green fluorescent protein) and Luciferase (firefly luciferase) genes. The pseudovirus is replication-defective and has the ability to infect 293T-ACE2 cells. Its infectivity can be measured through the detection of fluorescence and Luciferase activity.
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Overview