RNase Viability Assay Kit (Fluorescent Labeling)

RNase Viability Assay Kit (Fluorescent Labeling)

Catalog Number:
EAK1505817YEA
Mfr. No.:
41309ES96/97
Price:
$553
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      • Overview
        • The RNase Activity Detection Kit (Fluorescence Labeling Method) uses a new type of RNA substrate, which is labeled with a fluorescent reporter molecule (Fluor) at one end and a quencher at the other end. In the absence of RNase, the physical proximity of the quencher quenches the fluorescence in the fluorescent reporter to an extremely low level. When RNase is present, the RNA substrate is hydrolyzed, and the fluorescent reporter and quencher are spatially separated in the solution, which causes the Fluor to emit bright fluorescence when excited by light of the appropriate wavelength. This fluorescence can be detected by a multifunctional microplate reader (with a fluorescence module) and a fluorometer based on a filter or a monochromator.

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      • Properties
        • Details
          Components:
          RNase Substrate (1 tube/5×1 tube)
          10×RNase buffer (1 mL/5 mL)
          RNase A (≈1×10-4 U/μL) (100 μL/500 μL)
          DEPC-treated Water (10 mL/50 mL)
          Surface RNase Remover (50 mL/250 mL)
          Storage
          All components are shipped on dry ice and are valid for 1 year.
          After receiving the goods, please check whether all 5 components are complete, then store the 41309-E component at 2-8°C and the other components at -25~-15°C to avoid repeated freezing and thawing.

          * For Research Use Only

      • Applications
        • Application Description
          The RNase Activity Detection Kit (Fluorescence Labeling Method) uses a substrate fluorescence labeling method to quantitatively or qualitatively detect RNase in a single sample, thereby determining whether the sample is contaminated with RNase.

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