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Overview
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The RNase Activity Detection Kit (Fluorescence Labeling Method) uses a new type of RNA substrate, which is labeled with a fluorescent reporter molecule (Fluor) at one end and a quencher at the other end. In the absence of RNase, the physical proximity of the quencher quenches the fluorescence in the fluorescent reporter to an extremely low level. When RNase is present, the RNA substrate is hydrolyzed, and the fluorescent reporter and quencher are spatially separated in the solution, which causes the Fluor to emit bright fluorescence when excited by light of the appropriate wavelength. This fluorescence can be detected by a multifunctional microplate reader (with a fluorescence module) and a fluorometer based on a filter or a monochromator.
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Overview