Recombinant Staphylococcal Protein-A Long Form

Recombinant Staphylococcal Protein-A Long Form

Catalog Number:
P001414157ABE
Mfr. No.:
Abe32-4938
Price:
  • Size:
    100 mg
    Quantity:
    Add to Cart:
      • Overview
        • SPA Recombinant Long Form produced in E. Coli is a single non-glycosylated polypeptide chain. SPA Long Form is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 422 amino acids (including a.a 273-400 from acc# P99134) and having a molecular mass of 46.6kDa containing little or no carbohydrate. Protein A is a cell wall component produced by several strains of Staphylococcus aureus. The recombinant Protein A is genetically engineered protein and holds 5 IgG-binding regions of protein A. Recombinant Protein A functions basically the same as native Protein A and is perfect for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to human IgG1, IgG2 and IgG4, mouse IgG2a, IgG2b and IgG3 and rat IgG2c. Protein A also binds to total IgG from rabbit, pig, dog, cat, and guinea pig.

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      • Properties
        • Protein Name
          Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
          Formulation
          SPA protein was lyophilized with no additives.
          Storage
          Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized SPA in sterile 18MΩ-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.

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