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Recombinant Soy Bean P34 Protein, GST Tag

Recombinant Soy Bean P34 Protein, GST Tag

Catalog Number:
P001414576ABE
Mfr. No.:
Abe32-5356
Price:
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  • Size:
    0.5 mg
    Quantity:
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      • Overview

        • The E. Coli derived GST Tag recombinant protein, 36kDa, contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids. The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2-3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.

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      • Properties
        • Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Protein is >95% pure as determined by 10% PAGE (coomassie staining) and RP-HPLC.
          Formulation
          50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.
          Storage
          Soybean P34 His although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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