Name: Recombinant SARS-CoV-2 S1+S2 ECD (S-ECD) Protein His tag
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- Overview
  - Endotoxin < 0.1 EU/μg of the protein by LAL method. Recombinant 2019-nCoV S1+S2 ECD（S-ECD） Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence Cys15-Gln1208 (Pro986, 987)(682-685GSAS) of 2019-ncov S1+S2 ECD（S-ECD） (Accession #QHD43416.1) fused with a 6×His tag at the C-terminus.
    
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- Properties
  - Protein Name
    
    cov spike Protein; 2019-nCoV; ncov spike Protein; 2019-nCoV; coronavirus spike Protein; 2019-nCoV; S glycoprotein; COVID-19; S protein; Spike glycoprotein
    
    Source
    
    HEK293 cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    >97% by SDS-PAGE.
    
    Formulation
    
    Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
    
    Storage
    
    This product is stable at ≤ ‑70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
    
    More Information
    
    Gene ID
    
    43740568
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    Functional Assay
    
    Application Description
    
    Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1+S2 ECD at 2μg/mL (100 µl/well) can bind Recombinant Human ACE2 with a linear range of 0.15-3.03 ng/mL.

Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.

Recombinant SARS-CoV-2 S1+S2 ECD (S-ECD) Protein His tag

Recombinant SARS-CoV-2 S1+S2 ECD (S-ECD) Protein His tag

More Information

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