Name: Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein
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- Overview
  - Endotoxin < 1.0 EU/μg of the protein by LAL method. Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.
    
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- Properties
  - Protein Name
    
    S1 protein, Spike glycoprotein Subunit1, S glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein
    
    Source
    
    HEK293 cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    >95% by SDS-PAGE.
    
    Formulation
    
    Lyophilized from a 0.22 µm filtered solution of PBS, pH 7.4.
    
    Storage
    
    Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.
    
    Activity
    
    Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.
    
    More Information
    
    Gene ID
    
    43740568
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    Functional Assay
    
    Application Description
    
    Reconstitution: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 µl/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.

Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein

Recombinant Sars-Cov-2 (COVID-19/2019-nCov) Spike S1 Protein

More Information

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