Name: Recombinant IL-15, Human, GMP
Price: 1445.00 USD

- Overview
  - Interleukin-15 (IL-15) is a 14-15kDa glycoprotein with immune regulatory functions in many diverse cell types. IL-15 can be constitutively expressed in a variety of cell types stored as intracellular protein in the cytoplasm as well as transport to the cell surface, while only secreted from some cell types including monocytes, dendritic cells, epithelial cells, bone marrow stromal cells, and fibroblasts. As a pleiotropic cytokine, IL-15 mediates the crosstalk between innate immunity and adaptive immunity whose principal role is to kill virally infected cells. IL-15 plays a crucial role in the development, differentiation, and survival of NK cells. In monocytes, IL-15 induces the production of IL-8 and monocyte chemotactic protein 1 (MCP-1), which recruits neutrophils and monocytes to sites of infection. IL-15 can also act as a chemo-attractant in T lymphocytes and regulate the differentiation of T lymphocytes.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    interleukin 15, IL-T, IL15
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Reactivity
    
    Human
    
    Purification
    
    >95% as determined by SDS-PAGE analysis.
    
    Formulation
    
    Lyophilized from a 0.2µm filtered solution of PBS, pH 8.0.
    
    Storage
    
    Lyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protein aliquots should be stored at -20°C or -80°C for 3-6 months. Avoid repeated freeze/thaw cycles.
    
    Note
    
    Centrifuge at 3000 rpm for 5 mins before opening. It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 0.5 mg/mL and incubate the stock solution at RT for at least 20 mins to ensure sufficient re-dissolved. Do Not Vortex! Vigorous shaking may impair the biological activity of the protein.
    
    Molecular Weight
    
    The protein has a calculated MW of 13.7kDa. The protein migrates as 13kDa under reducing condition (SDS-PAGE analysis).
    
    Class
    
    Cytokines
    
    Sequence
    
    NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISLESGDASIHDTVENLIILANNSLSSNGNVTESGCKECEELEEKNIKEFLQSFVHIVQMFIN TS with polyhistidine tag at the N-terminus.
    
    Affinity Tag
    
    His Tag (N-term)
    
    Endotoxin
    
    <0.05EU per 1μg of the protein by the LAL method.
    
    Activity
    
    Measure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <3ng/mL. The specific activity of recombinant human IL-15 is >2x10^6 IU/mg, which is calibrated against the human IL-15 WHO International Standard (NIBSC code: 95/554).
    
    More Information
    
    UniProt
    
    P40933
    
    * For research use only.

Recombinant IL-15, Human, GMP

Recombinant IL-15, Human, GMP

More Information

We Also Recommend

Recombinant IL-18, Human, GMP

Recombinant IL-2, Human, GMP

Recombinant IL-21, Human, GMP