Name: Recombinant human PCMT1 protein , 1-227aa
Price: 240.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    Protein-L-isoaspartate D-aspartate O-methyltransferase, PIMT, L-isoaspartyl protein carboxyl methyltransferase, Protein L-isoaspartyl/D-aspartyl methyltransferase, Protein-beta-aspartate methyltransferase
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE
    
    Formulation
    
    Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl
    
    Storage
    
    Stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
    
    Concentration
    
    1mg/ml (determined by Bradford assay)
    
    Molecular Weight
    
    28.7 kDa (263aa) confirmed by MALDI-TOF
    
    Expression system
    
    E.coli
    
    More Information
    
    UniProt
    
    P22061
    
    * For research use only. Not intended for human, diagnostics or veterinary use.
- Applications
  - Application
    
    SDS-PAGE
- Reference
  - Maeda H, et al. Rapid survey of four Asp isomers in disease-related proteins by LC-MS combined with commercial enzymes. (Anal Chem. 2015)
    Lyon YA, et al. Differences in α-Crystallin isomerization reveal the activity of protein isoaspartyl methyltransferase (PIMT) in the nucleus and cortex of human lenses. (Exp Eye Res. 2018)
    Takata T, et al. Site-specific rapid deamidation and isomerization in human lens αA-crystallin in vitro. (Protein Sci. 2020)
    Magami K, et al. Isomerization of Asp is essential for assembly of amyloid-like fibrils of αA-crystallin-derived peptide. (PLoS One. 2021)

3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Recombinant human PCMT1 protein , 1-227aa

Recombinant human PCMT1 protein , 1-227aa

More Information

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