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Recombinant Human Non-Muscle Myosin-II Regulatory Light Chain

Recombinant Human Non-Muscle Myosin-II Regulatory Light Chain

Catalog Number:
P001413580ABE
Mfr. No.:
Abe32-4332
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  • Size:
    10 µg
    Quantity:
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      • Overview

        • Non-Muscle Myosin-II Regulatory Light Chain Human Recombinant full length expressed in E.coli. The NMM is purified by proprietary chromatographic techniques. Non muscle Myosins are required for cytokinesis at the end of cell division, when a contractile ring near the plasmalemma divides the cytoplasm of the daughter cells. They are involved in cytoplasmic streaming movements in tissues, and especially in activation of motile cells such as fibroblasts and macrophages. Activation of non-muscle myosin-II is achieved by phosphorylation of Ser33 in the motif KKRPQRATSN by a dedicated, Ca +2 Calmodulin regulated light chain kinase (MLCK).

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      • Properties
        • Protein Name
          Non-Muscle Myosin-II Regulatory Light Chain, NMM.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 95.0% as determined by SDS-PAGE.
          Formulation
          NMM protein at 0.2mg/ml in 20mM HEPES-KOH, pH7, 50mM NaCl, 1mM EDTA and 1mM DTT.
          Storage
          Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          Assayed for phosphorilation by MLCK.

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