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Recombinant Human NECAP Endocytosis Associated 2

Recombinant Human NECAP Endocytosis Associated 2

Catalog Number:
P001413556ABE
Mfr. No.:
Abe32-4309
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  • Size:
    20 µg
    Quantity:
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      • Overview

        • NECAP2 produced in E. Coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (1-263a.a.) and having a molecular mass of 30.5 kDa. NECAP2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. NECAP2 which is involved in endocytosis, is a vital protein paralogues for clathrin-mediated membrane trafficking which are enhanced in CCV layers. NECAP2 protein colocalizes with AP-2 at the plasma membrane by binding AP-2s -ear domain, and interacts with AP-1, AP-2 and several GAE domain proteins termed GGA1, GGA2 and GGA3.

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      • Properties
        • Protein Name
          NECAP endocytosis-associated protein 2, NECAP-2, FLJ10420, Adaptin ear-binding coat-associated protein 2.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 85% as determined by SDS-PAGE.
          Formulation
          The NECAP2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT and 10% glycerol.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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