Name: Recombinant Human Myosin Light chain, Phosphorylatable, Fast Skeletal Muscle
Price: Inquiry

- Overview
  - MYLPF produced in E. Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (1-169 a.a) and having a molecular mass of 21.2kDa. MYLPF is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Myosin regulatory light chains, including MRCL3, MYLPF and MYL9, regulate contraction in smooth muscle and non-muscle cells via phosphorylation by MLCK (myosin light chain kinase). Phosphorylation of myosin regulatory light chains, catalyzed by MLCK in the presence of calcium and calmodulin, increases the actin-activated myosin ATPase activity, thus regulating the contractile activity. MYLPF is vital for fast and slow skeletal muscle development.
    
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- Properties
  - Protein Name
    
    Myosin regulatory light chain 2 skeletal muscle isoform, Fast skeletal myosin light chain 2, MLC2B, MYLPF, MRLC2, MYL11, HUMMLC2B.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 85.0% as determined by SDS-PAGE.
    
    Formulation
    
    MYLPF protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 100mM NaCl.
    
    Storage
    
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Recombinant Human Myosin Light chain, Phosphorylatable, Fast Skeletal Muscle

Recombinant Human Myosin Light chain, Phosphorylatable, Fast Skeletal Muscle

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