Recombinant Human Myosin Light Chain 5

Recombinant Human Myosin Light Chain 5

Catalog Number:
P001413387ABE
Mfr. No.:
Abe32-4265
Price:
  • Size:
    20 µg
    Quantity:
    Add to Cart:
      • Overview
        • MYL5 Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (1-132 a.a.) and having a molecular mass of 17.4kDa. MYL5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Myosin regulatory light chain 5 (MYL5) is a hexameric ATPase cellular motor protein. Myosin is comprised of 2 heavy chains, 2 nonphosphorylatable alkali light chains, and 2 phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in the fetal muscle and in the adult retina, cerebellum, and basal ganglia. The reconstitution of myosin with MYL5 or alkali light chain increases filament velocity to intermediate rates, and the re-addition of both classes of light chains fully reinstates the original sliding pace.

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      • Properties
        • Protein Name
          Myosin light chain 5, Myosin regulatory light chain 5, Superfast myosin regulatory light chain 2, MYLC2, MyLC-2, MYL5.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 90.0% as determined by SDS-PAGE.
          Formulation
          MYL5 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol and 0.1M NaCl.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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