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Overview
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MB produced in E. Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids (1-154a.a.) and having a molecular mass of 19.3kDa. MB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Myoglobin, mitochondrial precursor is a cytosolic oxygen-binding protein in charge of the storage and diffusion of oxygen within myocytes. The largest expression of MB is in the skeletal and cardiac muscle. MB operates in various functions in muscular oxygen supply, such as oxygen storage, facilitated diffusion, and myoglobin-mediated oxidative phosphorylation.
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- Properties
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Overview