Name: Recombinant Human GroES (HSP10), His Tag
Price: Inquiry

- Overview
  - GroES His Protein is 12.0 kDa protein containing 111 amino acid residues of the GroES Human and the 10 aa N-Terminal His-tag. HSP10 is part of the molecular chaperons, that are crucial for thir efficient folding of proteins in normal as well as stress conditions. GroES function is to bind to HSP60 in the presence of ATP, thus causing a change in the HSP60 conformation & enclosing the protein substrate within the complex. ATP hydrolysis by chaperonin-60 which destabilizes the HSP10-HSP60 complex, thereby allowing it to dissociate and secrete the substrate protein. GroES having the NCBI accession number of NP_002148 was purified by using conventional chromatography techniques.
    
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- Properties
  - Protein Name
    
    CPN10, GROES, HSP10, HSPE1, Chaperonin-10, 10 kDa heat shock protein mitochondrial, 10 kDa chaperonin, Early-pregnancy factor, EPF.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 90% as determined by SDS-PAGE.
    
    Formulation
    
    GroES His Human was filtered (0.4µm) and lyophilized from 0.5 mg/ml supplied in 0.05M phosphate buffer, 0.075 M NaCl, pH 7.4.
    
    Storage
    
    Store lyophilized GroES His at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted GroES His can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application Description
    
    It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

Recombinant Human GroES (HSP10), His Tag

Recombinant Human GroES (HSP10), His Tag

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