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Recombinant Bacillus Circulans Intein

Recombinant Bacillus Circulans Intein

Catalog Number:
P001413269ABE
Mfr. No.:
Abe32-4019
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  • Size:
    20 µg
    Quantity:
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      • Overview

        • Intein Bacillus Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 533 amino acids (3-518) and having a molecular mass of 59.4 kDa. Intein is fused to a 16 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. Intein is a section of a protein which can remove itself and return the remaining segment with a peptide bond. In addition, Inteins hold an endonuclease domain which takes part in Intein proliferation. Actually, various genes have unrelated intein-coding segments inserted at altered positions and they were found in all three domains of life (eukaryotes, bacteria, and archaea) and in viruses.

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      • Properties
        • Protein Name
          Intein-CBD
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 95% as determined by SDS-PAGE.
          Formulation
          The Intein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol.
          Storage
          Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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