Name: Recombinant 2019-nCoV Spike RBD Protein His tag
Price: Inquiry

- Overview
  - Endotoxin: < 0.1 EU/μg of the protein by LAL method. Recombinant 2019-nCoV Spike RBD Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
    
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- Properties
  - Protein Name
    
    S1-RBD protein; NCP-CoV RBD Protein; novel coronavirus RBD Protein; 2019-nCoV RBD Protein; S glycoprotein Subunit1 RBD Protein
    
    Source
    
    HEK293 cells
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% by SDS-PAGE; > 95% by HPLC
    
    Formulation
    
    Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4 or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
    
    Storage
    
    Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. This liquid product is stable at ≤ ‑70°C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.
    
    More Information
    
    Gene ID
    
    43740568
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.
- Applications
  - Application
    
    Functional Assay
    
    Application Description
    
    Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike RBD Protein at 2μg/mL (100 µl/well) can bind Recombinant Human ACE2 with a linear range of 0.12-9.06 ng/mL.

Recombinant 2019-nCoV Spike RBD Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.

Recombinant 2019-nCoV Spike RBD Protein His tag

Recombinant 2019-nCoV Spike RBD Protein His tag

More Information

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