Raw264.7 Cell Lysate

Raw264.7 Cell Lysate

Catalog Number:
CL01443484NKM
Mfr. No.:
ATGL0026
Price:
$260
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      • Overview
        • Raw264. 7 cells are established from ascites of a tumour induced in a male mouse by intraperitoneal injection of Abselon Leukaemia Virus (A-MuLV). This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. Cells will pinocytose neutral red and phagocytose zymosan. Cells capable of antibody dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS.

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      • Properties
        • Categories
          Cell Lysates
          Formulation
          Liquid. In SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
          Concentration
          2mg/ml (determined by BCA assay)
          Other Properties
          Quality Control: Western Blot analyzed by both GAPDH and Beta tubulin antibody
          Storage
          Aliquot the lysate into single tube and then storing them -80°C or below. Avoid repeated freezing and thawing cycles.

          * For research use only. Not intended for human, diagnostics or veterinary use.

      • Applications
        • Application Description
          Heat lysate to 95°C for 5 minutes and rapidly cooling before use.

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