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QuantiChrom™ Phosphate Assay Kit

QuantiChrom™ Phosphate Assay Kit

Catalog Number:

HTSA373964BIO

Mfr. No.:

BIO-DIPI-500

Price:

$527

Size:

500 tests

Quantity:

Add to Cart:

- Overview
  - Phosphate (Pi) is one of the most important ion species in nature. Phosphate is present in all biological systems. It is a major constituent in minerals and fertilizers, and is a component of industrial wastewater. Thus accurate determination of phosphate concentration finds numerous applications in pharmacology, biomedical research, clinical chemistry, industrial process monitoring and environmental monitoring. Simple, direct and automation-ready procedures for measuring phosphate concentration in biological and environmental samples are becoming popular. Our phosphate assay kit is designed to measure phosphate ion directly in samples without any pretreatment. The improved Malachite Green method utilizes the malachite green dye and molybdate, which forms a stable colored complex specifically with inorganic phosphate. The intensity of the color, measured at 620nm, is directly proportional to the phosphate concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
    
    Key Features
    ▪ Sensitive and accurate. Linear detection range 0.3 µM (0.0028 mg/dL) to 50 µM (0.47 mg/dL) phosphate in 96-well plate assay.
    ▪ Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 30 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
    ▪ Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Assays can be executed in cuvet or 96-well plate.
    ▪ Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipid, protein and minerals.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Method: OD620nm (malachite green)
    Samples: Serum, urine, saliva, sweat, tissue culture, food, environment etc
    Species: All
    Procedure: 30 min
    Detection Limit: 3 µg/dL (0.3 µM)
    Shelf Life: 12 months
    
    Storage
    
    4°C
    
    * For Research Use Only
- Applications
  - Application Description
    
    For quantitative determination of phosphate and evaluation of drug effects on phosphate metabolism.
- Reference
  - Zhao, Z et al. (2020). Bacteria elevate extracellular adenosine to exploit host signaling for blood-brain barrier disruption. Virulence, 11(1): 980-994. Assay: Phosphate in Streptococcus suis culture.
    Onishi, A et al. (2020). A role for tubular Na+/H+ exchanger NHE3 in the natriuretic effect of the SGLT2 inhibitor empagliflozin. American Journal of Physiology. Renal Physiology, 319(4): F712-F728 Assay: Phosphate in mouse plasma and urine.
    Rimondi, E et al. (2021). Role of vitamin D in the pathogenesis of atheromatosis. Nutrition, Metabolism, and Cardiovascular Diseases, 31(1): 344-353 Assay: Phosphate in mouse serum.
    Shao, J et al. (2020). Photochemical activity of black phosphorus for near-infrared light controlled in situ biomineralization. Advanced Science (Weinheim, Baden-Wurttemberg, Germany), 7(14), 2000439. Assay: Phosphate in Black Phosphorus semiconductors.
    Ghayor, C et al. (2020). Microporosities in 3d-printed tricalcium-phosphate-based bone substitutes enhance osteoconduction and affect osteoclastic resorption. International Journal of Molecular Sciences, 21(23). Assay: Phosphate in Tricalcium phosphate scaffolds.
    Kim, MY et al. (2021). Metabolic activities affect femur and lumbar vertebrae remodeling, and anti-resorptive risedronate disturbs femoral cortical bone remodeling. Experimental & Molecular Medicine, 53(1): 103-114. Assay: Phosphate in mouse serum.
    Al-Saiedy, M., Gunasekara, L., Green, F., Pratt, R., Chiu, A., Yang, A. & Amrein, M. (2018). Surfactant Dysfunction in ARDS and Bronchiolitis is Repaired with Cyclodextrins. Military medicine, 183(suppl_1), 207-215. Assay: Phosphate in bovine lipid.
    Dai, J., Lai, L., Tang, H., Wang, W., Wang, S., Lu, C. & Wu, Z. (2018). Streptococcus suis synthesizes deoxyadenosine and adenosine by 5'-nucleotidase to dampen host immune responses. Virulence, 9(1), 1509-1520. Assay: Phosphate in mouse blood.
    Shamsuzzaman, S., Onal, M., St John, H. C., Jeffery, J. J., & Pike, J. W. (2017). Absence of the vitamin D receptor inhibits atherosclerotic plaque calcification in female hypercholesterolemic mice. Journal of cellular biochemistry, 118(5), 1050-1064. Assay: Phosphate in fish serum.
    Subramaniyan, S. A., Da Rae Kang, S. A. B., Eun-So-Ri Cho, J. H., Jung, Y. C. J., Choi, Y. I., & Shim, K. S. (2016). Meat quality and physicochemical trait assessments of berkshire and commercial 3-way crossbred pigs. Korean journal for food science of animal resources, 36(5), 641. Assay: Phosphate in pig tissue.
    Villa-Bellosta, R., Gonzalez-Parra, E., & Egido, J. (2016). Alkalosis and dialytic clearance of phosphate increases phosphatase activity: a hidden consequence of hemodialysis. PloS one, 11(7), e0159858. Assay: Phosphate in human plasma.
    Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: phosphate in fish plasma.
    Dunbar, DR. et al (2010). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in mouse urine.
    Galitzer H, et al (2010). Parathyroid cell resistance to fibroblast growth factor 23 in secondary hyperparathyroidism of chronic kidney disease. Kidney Int.77(3):211-8. Assay: Phosphate in rat serum.
    Ponda MP, et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis. 210(1):57-62. Assay: Phosphate in mouse tissue.
    Xie J et al (2010). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in Plant tissue.
    Dunbar, DR et al (2009). Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney. Physiol Genomics. 40(3):158-66. Assay: Phosphate in mouse urine.
    Hildebrand, J. et al (2009) Functional and energetic characterization of P-gp-mediated doxorubicin transport in rainbow trout (Oncorhynchus mykiss) hepatocytes Comp Biochem Physiol C Toxicol Pharmacol. 149(1):65-72. Assay: Phosphate in fish hepatocyte.
    O'Connor RD, et al (2009). Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone. 45(2):346-56. Assay: Phosphate in mouse tissue.
    Xie J, et al (2009). Comparison of media used to evaluate Rhizobium leguminosarum bivar viciae for phosphate-solubilizing ability. Can J Microbiol. 55(7):910-5. Assay: Phosphate in plant tissue.
    Abranches, J. (2008). CcpA regulates central metabolism and virulence gene expression in Streptococcus mutans. J Bacteriol. 190(7):2340-9. Assay: Phosphate in bacteria cellular ATPase.
    Jubeck B, et al (2008). Promotion of articular cartilage matrix vesicle mineralization by type I collagen. Arthritis Rheum. 58(9):2809-17. Assay: Phosphate in human collagen.
    Chung YI, et al (2007). Enhanced bone regeneration with BMP-2 loaded functional nanoparticle-hydrogel complex. J Control Release 121(1-2):91-9. Assay: Phosphate in rat bone.

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