qPCR Kit - HEK293 RESIDUAL DNA QUANTITATION KIT

qPCR Kit - HEK293 RESIDUAL DNA QUANTITATION KIT

Catalog Number:
PK01527913YOU
Mfr. No.:
YSL-qP-HEK-100
Price:
  • Size:
    100 reactions
    Quantity:
    Add to Cart:
      • Overview
        • This YouSeq kit has been designed to quantitate residual DNA from CHO in cell lines which are used in the production of biopharmaceutical products. This is a key impurity for clearance during the purification process. Regulatory guidance for products produced in cell culture makes it very clear that DNA content in the final product should be minimal.

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Kit contents HEK293 primer/probe
          set YouSeq Lyophilised TetraTM 2X qPCR MasterMix
          Positive control/DNA standards MasterMix
          resuspension buffer
          RNase/DNase free water
          Template resuspension buffer
          ROX passive reference

          The YouSeq real-time PCR protocol is fast and easy to set up; less than 30 minutes of hands-on time. 45 cycles of qPCR are then performed in around 90 minutes delivering precise, reliable results in less than 2 hours.
          The HEK293 kit is designed and tested to provide highly specific detection of the cell line DNA species of interest and will not cross-react with any other species.

          * For Research Use Only. Not intended for diagnostic use.

      • Applications
        • Application Description
          Accurate, reliable screening for contaminating residual host cell DNA
          To screen for contaminating residual host cell DNA it is critical to use the most sensitive technology possible. Traditional methods have proven laborious and lack sensitivity and specificity. The YouSeq HEK293 Residual DNA Quantitation kit resolves all of these issues.

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