qIFNG Recombinant Protein

qIFNG Recombinant Protein

Catalog Number:
P001412066ABE
Mfr. No.:
Abe32-1329
Price:
  • Size:
    20 µg
    Quantity:
    Add to Cart:
      • Overview
        • Recombinant Equine Interferon-gamma produced in E. Coli is a single, non-glycosylated, polypeptide chain containing 143 amino acids and having a molecular mass of 16.7kDa. The IFN-gamma Equine is purified by proprietary chromatographic techniques. IFN-gamma produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

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      • Properties
        • Protein Name
          Interferon gamma, IFN-gamma, IFNG.
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
          Formulation
          Lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 5% trehalose.
          Storage
          Lyophilized Interferon-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-gamma should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

          * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

      • Applications
        • Application Description
          It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 10.0 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.

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