-
-
Overview
-
tRNALys1,2 is a primer of reverse transcriptase of human and simian spuma retroviruses, Visna virus, CAEV, MPMV, squirrel monkey retrovirus and EIAV. tRNALys1,2 is purified from the human placenta using the following procedures:
(1) the extraction of total RNA by phenol-chloroform; (2) the chromatogram extraction of total tRNA with DE52; (3) the chromatogram separation of total tRNALys with DEAE-Sephadex (A50); Detection is performed by aminoacylation of tRNA with H3-lableled lysine using aminoacyl tRNA synthetase from bovine liver. For long-term storage (years), it is recommended that this product be stored at -80°C at a concentration of no less than 0.5μg/μl. For short-term storage, this product is stable at -20°C for a minimum of one year.
Source: human placenta.
Purity: HPLC grade.Please contact us at for specific academic pricing.
Background
RNA is most widely used as the primer of retroviral reverse transcriptases. The 3’ end of the transfer RNA binds to the complementary binding site on the viral RNA therefore initiating reverse transcription.
tRNA Lysine 3 is the primer of HIV-I, HIV-II, SIV and FIV.
tRNA Lysine 1,2 are the primers of BLV, HTLV-I and II, SSV, FLV, VV, CAEV, MPMV, SMR and EIAV.
tRNA Tryptophan and tRNA Proline are the primers of ASNV, SSV, FLV, BLV, MLV, HTLV-I and II.
Since it has been well documented that artificial transfer RNA does not posses the same biological properties as natural tRNA, human purified transfer RNAs are largely in demand for retroviral research, especially HIV related research.
The extraction and purification of transfer RNA from a natural source such as human placenta is a highly complex and delicate procedure.
-
- Properties
- Applications
-
Overview