Login / Register

0

pSpark® Universal DNA Cloning Kit

pSpark® Universal DNA Cloning Kit

Catalog Number:

DC01268457CAN

Mfr. No.:

C0019

Price:

$418

Size:

20 rxn

Quantity:

Add to Cart:

- Overview
  - pSpark® Universal is a highly efficient, accurate and easy-to-use DNA Cloning kit ideal for a broad range of PCR fragments cloning applications. There is a range of DNA Polymerases available that do not generate PCR products with identical ends: proofreading DNA polymerases leave blunt ends while blends of polymerases and non-proofreading DNA polymerases leaves 3´A overhangs. Therefore, it is necessary to employ different vectors to clone both kinds of PCR fragments. pSpark® Universal DNA cloning kit has been designed to save time, looking for a kit for several cloning scenarios. It is mainly composed of two cloning vectors which allow blunt or TA DNA cloning. For blunt DNA cloning and TA DNA cloning, pSpark® II DNA Cloning vector and pSpark® TA DNA Cloning vector, respectively, are included.
    
    Includes for 20 rxn:
    – 20 µL pSpark® II (20 ng/µL)
    – 20 µL pSpark® TA (50 ng/µL)
    – 20 µL T4 DNA Ligase (5U/Weiss)
    – 200 µL T4 DNA Ligase Buffer (5x)
    – 150 µL PEG 6000 (10x)
    – 1 kb Insert Control (20 ng/µL)
    – Insert Control TA (30 ng/µL)
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    -20 °C (NON Frost-Free Freezer).
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application Description
    
    Cloning of high fidelity PCR amplified products into pSpark® II Blunt DNA cloning vector.
    Cloning of non-proofreading PCR fragments into pSpark® TA DNA Cloning vector.
    Production of ssDNA.
    In vitro transcription from T7/SP6 dual-opposed promoters.
- Reference
  - Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.
    Benítez Casanova, L. (2017). Caracterización de las principales enzimas celulolíticas de myceliophthora thermophila implicadas en la degradación de biomasa lignocelulósica y mejora de la hidrólisis de hemicelulosa para la producción de bioetanol de segunda generación.
    Oliveras Rovira, À., Baró, A., Montesinos Barreda, L., Feliu Soley, L., & Planas i Grabuleda, M. (2018). Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens. PLoS ONE, 2018, vol. 13, núm. 7, p. e0201571.
    Folgueira, I., Lamas, J., De Felipe, A. P., Sueiro, R. A., & Leiro, J. M. (2019). Evidence for the role of extrusomes in evading attack by the host immune system in a scuticociliate parasite. BioRxiv, 607622.
    Olmos, G., Martínez‐Miguel, P., Alcalde‐Estevez, E., Medrano, D., Sosa, P., Rodríguez‐Mañas, L., … & López‐Ongil, S. (2017). Hyperphosphatemia induces senescence in human endothelial cells by increasing endothelin‐1 production. Aging cell, 16(6), 1300-1312.
    Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutierrez, S. (2019). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology, 122, 31-46.
    Oliveras, À., Baró, A., Montesinos, L., Badosa, E., Montesinos, E., Feliu, L., & Planas, M. (2018). Antimicrobial activity of linear lipopeptides derived from BP100 towards plant pathogens. PloS one, 13(7), e0201571.
    Álvarez-Cao, M. E., Cerdán, M. E., González-Siso, M. I., & Becerra, M. (2019). Bioconversion of Beet Molasses to Alpha-Galactosidase and Ethanol. Frontiers in microbiology, 10.
    Álvarez-Escribano, I., Vioque, A., & Muro-Pastor, A. M. (2018). NsrR1, a nitrogen stress-repressed sRNA, contributes to the regulation of nblA in Nostoc sp. PCC 7120. Frontiers in microbiology, 9, 2267.
    TC, G. Supplemental Material. González-‐Aguilera C. et al.
    Aliaga‐Guerrero, M., Paullada‐Salmerón, J. A., Piquer, V., Mañanós, E. L., & Muñoz‐Cueto, J. A. (2017). Gonadotropin‐inhibitory hormone in the flatfish, Solea Senegalensis: Molecular cloning, brain localization and physiological effects. Journal of Comparative Neurology.
    Reñé, A., Alacid, E., Figueroa, R. I., Rodríguez, F., & Garcés, E. (2016). Life-cycle, ultrastructure, and phylogeny of Parvilucifera corolla sp. nov.(Alveolata, Perkinsozoa), a parasitoid of dinoflagellates. European Journal of Protistology.
    Paullada‐Salmerón, J. A., Cowan, M., Aliaga‐Guerrero, M., Gómez, A., Zanuy, S., Mañanos, E., & Muñoz‐Cueto, J. A. (2016). LPXRFa peptide system in the European sea bass: a molecular and immunohistochemical approach. Journal of Comparative Neurology, 524(1), 176-198.

Documents

Note: If you don't receive our verification email, do the following:

Confirm that you entered your email address correctly.
Check if the email is in your spam or junk folder.
Or you may contact us at .

Products

Amerigo Scientific

Support

Contact Us

Copyright © Amerigo Scientific. All rights reserved.