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Overview
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pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.
Includes for 20 rxn:
– 20 µL pSpark® IV (20 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 200 µL T4 DNA Ligase Buffer (5x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)Please contact us at for specific academic pricing.
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- Properties
- Applications
- Reference
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Overview