Pseudo-UTP

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Pseudo-UTP, also known as pseudouridine-5’-O-triphosphate, is one of the most commonly used modified nucleoside-5’-O-triphosphates. Pseudo-UTP is a building block for the polymerase-mediated synthesis of RNA molecules. RNA molecules, especially messenger RNAs (mRNAs), have widespread applications in therapeutic and diagnostic areas, and have demonstrated their superiority over plasmid DNA techniques in both gene and vaccination therapy. Compared with uridine-containing unmodified mRNAs, the application of pseudouridine-containing modified mRNAs exhibits better nuclease stability, immunogenicity, and translational properties. Thus, pseudo-UTP could be used to develop drugable mRNAs with improved properties, by replacing the standard bases by modified ones.
    Reference:
    1. Shanmugasundaram M, Senthilvelan A, Kore AR. Gram-scale chemical synthesis of base-modified ribonucleoside-5'-O-triphosphates. Current Protocols in Nucleic Acid Chemistry, 2016, 67: 13.15.1-13.15.10.
- Properties
  - Categories
    
    A modified nucleoside-5’-O-triphosphate, commonly used for drugable mRNA synthesis
    
    Alternative Name
    
    Pseudouridine-5'-Triphosphate, 5-Ribosyl Uracil; lithium (5-(2,4-dioxo-1,2,3,4-tetrahydropyrimidin-5-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl triphosphate
    
    Molecular Formula
    
    C9H15N2O15P3 (free acid)
    
    Molecular Weight
    
    484.1 (free acid)
    
    Appearance
    
    Solution
    
    Purity
    
    Purity ≥ 97% by AX-HPLC
    
    Storage
    
    Store at -20°C or below
    
    SMILES
    
    O=C(NC=C1C2C(O)C(O)C(COP([O-])(OP([O-])(OP([O-])([O-])=O)=O)=O)O2)NC1=O.[Li+].[Li+].[Li+].[Li+]
    
    * For Research Use Only
- Reference
  - 1. Shudan Tan, Xue Hu, et al. "Preclinical evaluation of RQ3013, a broad-spectrum mRNA vaccine against SARS-CoV-2 variants." biorxiv
    2. Wei Wang, Shuo Feng, et al. "Prediction of lipid nanoparticles for mRNA vaccines by the machine learning algorithm." Acta Pharm Sin B.
    3. Gang Wang, Juan Shi, et al. "mRNA vaccines elicit potent neutralization against multiple SARS-CoV-2 omicron subvariants and other variants of concern." iScience. PMID: 36471872
    4. Kyusik Q Kim, Bhagyashri D Burgute, et al. "N1-methylpseudouridine found within COVID-19 mRNA vaccines produces faithful protein products." Cell Rep. PMID: 35988540
    5. Yao Li, Xiaotu Ma, et al. "Rapid Surface Display of mRNA Antigens by BacteriaDerived Outer Membrane Vesicles for a Personalized Tumor Vaccine." Adv Mater. 2022 May;34(20):e2109984. PMID: 35315546
    6. Jingjing Zhang, Yun Li, et al. "Genome-wide CRISPR/Cas9 library screen identifes PCMT1 as a critical driver of ovarian cancer metastasis." J Exp Clin Cancer Res. PMID: 35033172
    7. Trine I Jensen, Nanna S Mikkelsen, et al. "Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi." Genome Res. PMID: 34407984
    8. Cecilia Martinez Campos, Kevin Tsai, et al. "Mapping of pseudouridine residues on cellular and viral transcripts using a novel antibody-based technique." RNA. PMID: 34376564
    9. Jing Lu, Guoliang Lu, et al. "A COVID-19 mRNA vaccine encoding SARS-CoV-2 virus-like particles induces a strong antiviral-like immune response in mice." Cell Res. 2020;1-4. PMID:32801356

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Pseudo-UTP

Background

Documents

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