Proteinase K

- Overview
  - Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. It is a serine protease that exhibits broad cleavage specificity. With a molecular weight 28. 900 kD, it cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. It is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12. 5).
    
    Includes for 30 mg:
    – 30 mg Proteinase K (Lyophilized powder)
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Proteinase K Solution
    
    Storage
    
    4 °C or -20 °C.
    
    * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.
- Applications
  - Application
    
    End-Point PCR
    
    Application Description
    
    Isolation of genomic DNA from cultured cells and tissues.
    Removal of DNases and RNases during DNA and/or RNA purification.
    Determination of enzyme locations.
- Reference
  - Chekli, Y., Peron-Cane, C., Dell’Arciprete, D., Allemand, J. F., Li, C., Ghigo, J. M., … & Beloin, C. (2020). Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Scientific reports, 10(1), 1-13.
    Merinas-Amo, R. (2021). Papel de aditivos alimentarios en la modulación de procesos degenerativos: estudios in vivo e in vitro.

Datasheet

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Brochure

Proteinase K

Documents

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