Protein A Coated - 96 well plates - 8 Well Strips on 12 x 8 frame

Protein A Plates 96-Well Strip Plates: Designed with 12 strips of 8 wells on a rigid frame, preventing misorientation for consistent and accurate assays.

Protein A Plates are specifically designed to capture IgG, either directly or as part of antibody/antigen complexes. The Protein A surface offers an alternative to traditional passive adsorption methods for immobilizing antibodies in ELISA and other plate-based assays. Protein A binds specifically to the Fc region of immunoglobulins from various mammalian species, leaving the F(ab)2 binding sites free for efficient interaction with epitopes.

The Protein A Coated 96-Well Plates enable specific and sterically oriented binding of IgG, making them ideal for a variety of applications. These include the separation of IgG from other immunoglobulins or contaminants, isolation of antigen-antibody complexes, and the analysis and isolation of fusion proteins. Additionally, the plates can be used for the detection and identification of red cell antibodies, particularly when using U-bottom plates, providing a versatile tool for advanced immunoassays and protein analysis.

Protein A Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Protein A Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Protein A Coated Surface on Protein A Coated 96 Well Solid Plates is specifically designed to capture IgG, either directly or as part of antigen/antibody complexes. These plates are ideal for applications such as the specific and sterically oriented binding of IgG, the separation of IgG from other immunoglobulins or contaminants, the isolation of antigen-antibody complexes, and the analysis of fusion proteins. This versatile platform ensures efficient and reliable results in immunoassays requiring precise IgG binding and separation.

Key features of Protein A Plates
· Protein A surface: Captures IgG, either directly or as part of antibody/antigen complexes.
· Specific and sterically oriented binding of IgG
· Separation of IgG from other immunoglobulins or contaminants
· Isolation of antigen-antibody complexes
· Analysis and isolation of fusion proteins
· Detection and identification of red cell antibodies (applicable on U-bottom plates)
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Protein A Coated Surface is designed for the capture of IgG applied directly or as an antibody/antigen complex.
Protein A Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.

Protein A specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated on a surface, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.

· Examples of applications for Protein A Coated surface
specific and sterically oriented bond of IgG
separation of IgG from other immunoglobulins
separation of antigen-antibodies complexes
separation of IgG from contaminants
isolation and analysis of fusion proteins
finding and identifying red cell antibodies (only on U-bottom plates) (see Test for finding and identifying red cell antibodies by means of solid phase method)

· Protein A Coated Surfaces main features
Biomat Protein A Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Protein A Coated Products ensures:

Uniformity
The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Protein A Coated Microplates Specifications
Coating
Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 200 µl/well.

Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

Sensitivity
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Protein A Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 100 µl/tube.