PRImeDETECT™ Salmonella Detection Kit

PRImeDETECT™ Salmonella Detection Kit

Catalog Number:
PK01268551CAN
Mfr. No.:
FP0020; FP0021
Price:
$490
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      • Overview
        • PRImeDETECT™ Salmonella Detection Kit (Salmonella spp) is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp by the real-time PCR method. The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors. Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM, whereas the probe for the detection of IAC is labelled with the JOE fluorochrome. In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.

          Includes for 48 rxn:
          – 1 x PCR MasterMix
          – 1 x DNAready Lysis Buffer
          – 1 x PCR Positive Control
          – 1 x PCR Negative Control

          Please contact us at for specific academic pricing.

      • Properties
        • Details
          Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%).
          Quantification limit: 20 UG per reaction (100%).
          Quantification Dynamic range: 6 logs.
          Target: Salmonella spp.
          Inclusivity: 100% Inclusivity. 700 Salmonella strains tested, including S.enterica and S. bongori
          Exclusivity: 100%. Tested with 42 strains of the rest of Enterobacteriaceae and Eubacteria
          Detection:Probe labelled with fluorescent dyes Salmonella spp: FAM-BHQ1; IAC: HEX-BHQ1.
          Thermal cCycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.
          Storage
          -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.

          * This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals.

      • Applications
        • Application
          PCR Detection

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