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Overview
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PolyWAX LP™ is a silica-based material with an adsorbed, cross-linked coating of linear polyethyleneimine. The capacity is high for both small and large solutes.
PolyWAX LP™ is a hydrophilic weak anion-exchange (WAX) material developed by PolyLC for HPLC of enzymes and other proteins. Selectivity is excellent, with high or quantitative recovery of applied biological activity. Most anion-exchange materials based on polyethyleneimine (PEI) are prepared with the conventional branched polymer. PolyWAX LP™ is prepared with linear PEI, which confers greater selectivity and recovery.
The Use of PolyWAX LP™
▪ Purification of acidic proteins and polypeptides from natural products
▪ Analysis and purification of oligonucleotides and their analogs as well as amplified PCR products
▪ In proteomics, predigest fractionation of intact proteins via mixed-bed ion-exchange
▪ Anion-exchange of small, acidic solutes
For small solutes, use our 100-Å material. For peptides, use 300 Å. For proteins > 20 KDa, we recommend pore diameters of at least 1000 Å for optimal selectivity and efficiency. Our 3-µm, 1500-Å material affords superior separations of closely-related protein variants.Please contact us at for specific academic pricing.
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- Properties
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Overview