Polymyxin B ReadyMade™ Solution

1405-20-5

C55H96N16O13 x 2H2SO4

1385.61

Clear to yellow solution

Purity Level: >6000 u/mg (Dried Basis)

Mechanism of Action: Polymyxin B targets and alters the permeability of lipopolysaccharide (LPS) found in Gram-negative bacteria leading to lysing of the cell. Polymyxin B only needs to interact with LPS, it is not required to enter the cell.

2-8°C

Spectrum: Polymyxin B Sulfate has activity against Gram-positive bacteria, Gram-negative bacteria especially Pseudomonas aeruginosa, and fungi.

Microbiology Applications:
Polymyxin B Sulfate is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
Pseudomonas aeruginosa 0.25 µg/mL – 1 µg/mL
For a representative list of Polymyxin B Sulfate MIC values, click here.

Media Supplements:
Polymyxin B is routinely used as a selection agent in several types of isolation media:
Perfringens agar - Supplement A and Supplement B
Perfringens Agar - SFP and TSC Selective Supplements
Columbia Blood Agar - Campylobacter Selective Supplement (Skirrow)
Columbia Blood Agar - Campylobacter Selective Supplement (Blaser-Wang)
Brucella medium - Brucella Selective Supplement
MYP Agar - Polymyxin B Bacillus Selection Supplement
Legionella CYE Agar - Legionella BMPA-α Selective Supplement
Legionella CYE Agar - Legionella MWY Selective Supplement
Campylobacter Agar - Campylobacter Selective Supplement (Preston)
PALCAM Agar - PALCAM Selective Supplement
Legionella CYE Agar - Legionella GVPC Selective Supplement
m-CP Medium - Membrane C. perfringens Selective Supplement
Burkholderia cepacia Agar Base - Burkholderia cepacia Selective Supplement
ORSAB - ORSAB Selective Supplement
Campylobacter Agar Base - Modified Preston Campylobacter Selective Supplement
Brucella Medium Base - Modified Brucella Selective Supplement
Legionella CYE Agar - Legionella GVPN Selective Supplement
Chromogenic Listeria Agar - Chromogenic Listeria Selective Supplement
Chromogenic Bacillus cereus Agar - Chromogenic Bacillus cereus Selective Supplement
Chromogenic Listeria Agar - Chromogenic Listeria Differential Supplement

Plant Biology Applications:
Polymyxin B Sulfate was successfully tested to counteract phytopathogenic Gram-negative bacterial growth including different strains of Pseudomonas viridiflava and Erwinia carotovora. Polymixin B Sulfate was shown to reduce bacterial growth of different strains of Pseudomonas viridiflava at low concentrations, (0.08 µg/ml) and Erwinia carotovora growth at slightly higher concentrations (0.25 µg/ml) (Selim et al. 2005). Polymyxin B has been shown to elict alkaloid accumulation in E. californica. A concentration of 0.04 mg/ml for 4 hours showed a 5.5x increase in Jasmonate levels.