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Overview
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For Hydrophilic Interaction and Size Exclusion HPLC
This neutral, polar material is developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A™ affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.
The Use of PolyHYDROXYETHYL A™
▪ Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts
▪ Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.)
▪ Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests
▪ HPLC of solutes that aren't soluble in aqueous media (membrane proteins; phospholipids)
▪ Eliminating detergents, lipids, and salts from a sample
▪ Oligonucleotides and their analogs
In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode.
For proteins and peptides, use 200- or 300-Å material. For polar small solutes, try our premium 3-µm, 100-Å material.Please contact us at for specific academic pricing.
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- Properties
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Overview