Name: POLB Recombinant Protein
Price: Inquiry

- Overview
  - POLB Human Recombinant produced in E. Coli is a single, non-glycosylated polypeptide chain containing 355 amino acids (1-335 a.a) and having a molecular mass of 40.3kDa. POLB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. DNA polymerase beta (POLB) is a member of the DNA polymerase type-X family. In eukaryotic cells, POLB performs base excision repair necessary for DNA maintenance, replication, recombination, and drug resistance. POLB has 2 separate domains; the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity which excises the abasic sugar-phosphate residue at the strand break. POLB fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells. POLB overexpression, as seen in some human tumors, could convene an increase in spontaneous mutagenesis.
    
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- Properties
  - Protein Name
    
    DNA polymerase beta, POLB.
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    Greater than 90.0% as determined by SDS-PAGE.
    
    Formulation
    
    POLB protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl.
    
    Storage
    
    Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
    
    * For Research Use Only. Not for use in diagnostic/therapeutics procedures.

POLB Recombinant Protein

POLB Recombinant Protein

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