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Overview
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pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase. These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. Quote request
Includes for 20 rxn:
– 20 µL pMBL™ T-Vector (50 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600 bp (30 ng/µL)Please contact us at for specific academic pricing.
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- Properties
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Overview