PLD Cell-free Protein Expression Kit

Compared to traditional cell-based expression methods, cell-free protein expression is an excellent solution to accelerate research. The cell-free expression method can save costs and flexibly adjust reaction parameters to obtain target protein products in a faster and more efficient manner.
Amerigo Scientific offers PLD cell-free protein expression kits that rely on T7 RNA polymerase transcription with endogenous Escherichia coli (E. coli) translation system to synthesize recombinant proteins. The PLD kit contains all the components required by the transcription, translation, protein folding, and energy regeneration, such as the ribosome, tRNA, molecular chaperone, metabolic enzymes, translation initiation factors, and elongation factors.
PCR products and circular plasmids can be as templates for PLD cell-free protein expression kit. Fast and efficient expression of the target protein can be achieved simply by adding the template.

Features
• Fast
The protein expressed using our PLD kit can be detected after 1 hour, and can reach the peak at about 10 hours.
• Flexible
Our PLD kits support plasmids and PCR products as templates.
• High expression
Protein production yield above 3mg/mL can be achieved.
• Wide application
Our PLD kits can be used to express various types of proteins including proteins rich in disulfide bonds, membrane proteins, etc.
• Easy operation
Target proteins can be expressed after a one-step reaction by mixing reaction components in the kit with DNA template.
• High throughput
The PLD system can be used for high-throughput expression and screening in 96-well plates. Once the target protein is detected, the expression can be amplified.
• Protein co-expression
Multiple proteins can be expressed at the same time in the same reaction.

Kit Components
The PLD cell-free protein expression kit provides a high-efficient solution for preparing recombinant proteins with easy detection and purification. The kit contains:
• Optimized E. coli extract: enhancing the stability of the structure during DNA and increasing the yield of soluble proteins.
• Optimized reaction buffer: continuous energy supply for protein synthesis via ATP regeneration system.
• Optimized concentration ratios of amino acids: adequate substrate supply for protein synthesis.
• Optimized vector containing GFP gene: expressing green fluorescent protein as a positive control.

Please contact us at for specific academic pricing.