PickMutant™ Error Prone PCR Kit

PickMutant™ Error-Prone PCR Kit is based on the protocol of Cadwell and Joyce (1992). epPCR takes advantage of the inherently low fidelity of Taq DNA Polymerase (with errors biased toward AT to GC changes), which may be further decreased by the addition of Mn2+, increasing the Mg2+ concentration, and using unequal dNTP concentrations. The rate of Mutagenesis achieved by error-prone PCR is in the range of 0. 6-2. 0 %.

Includes for 30 rxn:
– 50 μL Taq Polymerase (5 U/μL)
– 200 μL 10x ep-PCR Buffer
– 80 μL ep-dNTP Mix (unbalanced dNTP ratio)
– 200 μL MnCl2 Solution (25mM)
– 2 x 1. 5 mL Water (PCR Grade)

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