PickMutant™ DNA Shuffling Kit

PickMutant™ DNA-Shuffling Kit is based on the method Developed by Stemmer. Stemmer introduced DNA shuffling, the first homologous recombination method, in 1994. DNA shuffling involves the digestion of a gene by DNase I into random fragments, and the reassembly of those fragments into a full-length gene by primerless PCR: the fragments prime on each other based on sequence homology, and recombination occurs when fragments from one copy of a gene anneal to fragments from another copy, causing a template switch, or crossover event. While randomly recombining the DNA sequences, the technique also introduces new point mutations at a relatively high rate (0. 7%). DNA shuffling consists of four steps: (i) preparation of genes to be shuffled, (ii) fragmentation with DNase I, (iii) reassembly by thermocycling in the presence of a DNA polymerase, and (iv) amplification of reassembled products by a conventional PCR.

Includes for 30 rxn:
– 40 μL Taq Polymerase (5 U/μL)
– 500 μL PCR Buffer (10x)
– 80 μL dNTP Mix (10 mM)
– 100 μL DNase I Solution (0. 1 U/ μL)
– 200 μL Reaction Buffer (10x)
– 100 μL Stop Solution
– 3 x 1. 5 mL PCR-grade Water

Please contact us at for specific academic pricing.